- Recombinant DNA - mixture of human gene and bacterial plasmid
- it is necessary to transfer the structure into the host cell
- in this instance, the host is a virus and inside is the nucleic acid such as DNA or RNA and around it is the protein cell
- the first thing is: remove the nucleic acid from the virus. Only the capsid protein shell is needed
- the plasmids are taken up by the virus and acts as a vector recombinant DNA. It is going to help transfer the DNA into the host cell.
- the reason why we choose the virus is because the virus, known as the phage, infects bacterial cells.
- it is able to attach to the cell membrane of the bacteria and insert the recombinant DNA into the host cell.
- at the end of the process, we have a bacterial cell which now contains the recombinant DNA including the human gene for insulin.
- the bacteria has its own DNA plus DNA from another organism. This combination is known as 'Transgenic'
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